AIM : Oxidation of Acetyl CoA to CO2 and H20. Also connectiong almost all individual metabolic pathways.
LOCATION:Mitochondrial matrix
FUNCTION: Generates high amount of NADH/FADH2 that act as fuel for ATP synthesis in ETC.
REQUIREMENT: Aerobic conditions only (NADH and FADH2 can be regenerated in ETC only in presence of oxygen)
REACTIONS IN KREB’S CYCLE
(Citrate is Kreb’sStarting Substrate ForMaking Oxaloacetate) –read more
Enzymes Mnemonics
So — citrateSynthetase
At —Aconitase
Another —Aconitase
Dance —isocitrate Dehydrogenase
Devon — alpha-ketoglutarate Dehydrogenase
Sipped — Succinyl-CoASynthetase
Down — Succinate Dehydrogenase
Five —fumarase
Drinks — malate dehydrogenase
Regulation of TCA:
Sr. No. | Regulating Enzyme | Inhibitred by | Activated by |
1. | Citrate Synthase | ATP NADH Acetyl CoA SuccinylCoa |
———– |
2. | Isocitrate dehydrogenase | ATP NADH | ADP |
3. | Alpha-Ketoglutarate | Succinyl CoA NADH |
———– |
Availability of ADP is also one of the regulating factors.
Krebs cycle is both catabolic and anabolic in nature , hence regarded as amphibolic
Reactions associated are:
- Oxaloacetate →Synthesis of Aspartate
- Alpha-Ketoglutarate → Synthesis of Glutamate
- Succinyl CoA → Synthesis of Porphyrins and Heme
- Mitochondrial Citrate →cytoplasm → cleaved to get acetyl CoA → Synthesis of fatty acids , sterols , etc..
Ox — Oxaloacetate
Such As — Aspartate
Keto — alpha-Ketoglutatrate
And
Gluta — Glutamate
Succeded —Succinyl CoA
Merrily — Mitochondrial
In
Proper — Porphyrin
Blood shed — Heme
In
Canada — Citrate