PCR is a technique for generating large quantities of specified DNA.
We doctors (DRs) should know the steps of Polymerase chain reaction (PCR). Hence, the steps are :
R- Renaturation (or annealing)
Now let us understand a little about what happens in the individual steps.
Denaturation is when the DNA gets denatured and the two strands separate. It takes 1 minute and requires a temperature of 95°C.
Renaturation, in which the primers bind with target DNA (by complementary base pairing). It takes 1 minute and requires a temperature of 55°C.
Synthesis in which primers are extended by joining complementary bases to DNA. It takes 2 minutes and requires a temperature of 75°C.